Development and of Diagnostic System for Detection of Cowpea chlorotic mottle virus using by Nested PCR
نویسندگان
چکیده
منابع مشابه
Versatile post-functionalization of the external shell of cowpea chlorotic mottle virus by using click chemistry.
We present the modification of the outer protein shell of cowpea chlorotic mottle virus (CCMV) with linear and strained alkyne groups. These functionalized protein capsids constitute valuable platforms for post-functionalization via click chemistry. After modification, the integrity of the capsid and the reversible disassembly behavior are preserved.
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The structure of cucumber mosaic virus (CMV; strain Fny) has been determined to a 3.2-A resolution using X-ray crystallography. Despite the fact that CMV has only 19% capsid protein sequence identity (34% similarity) to cowpea chlorotic mottle virus (CCMV), the core structures of these two members of the Bromoviridae family are highly homologous. As suggested by a previous low-resolution struct...
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Dispensability of 3' tRNA-like sequence for packaging cowpea chlorotic mottle virus genomic RNAs.
The 3' ends of three genomic RNAs (gRNAs) of cowpea chlorotic mottle virus (CCMV) terminate in a highly conserved tRNA-like structure (3'TLS). To examine the intrinsic role played the 3'TLS in packaging, the competence of each gRNA lacking the 3' TLS (DeltaTLS-gRNA) to interact with dissociated coat protein (CP) subunits and form virions was assayed in vitro. In contrast to the well established...
متن کاملFeasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations
BACKGROUND & METHODS Within the last decade Virus-Like Particles (VLPs) have increasingly received attention from scientists for their use as a carrier of (peptide) molecules or as scaffold to present epitopes for use in subunit vaccines. To test the feasibility of Cowpea chlorotic mottle virus (CCMV) particles as a scaffold for epitope presentation and identify sites for epitope fusion or inse...
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ژورنال
عنوان ژورنال: Korean Journal of Agricultural Science
سال: 2014
ISSN: 2466-2402
DOI: 10.7744/cnujas.2014.41.4.335